《植物生理学报》 2015, 51(12): 2114-2118

大麦赤霉病抗性种质的挖掘及其小孢子对DON毒素胁迫培养的响应

高润红^1,2, 郭桂梅^1,2, 何婷^1,2, 陈志伟^1,2, 徐红卫^1,2, 李颖波^1,2, 刘成洪^1,2, 陆瑞菊^1,2,*, 黄剑华^1,2,*

¹上海市农业科学院生物技术研究所, 上海201106; ²上海市农业遗传育种重点实验室, 上海201106

通信作者：陆瑞菊；E-mail: sw1@saas.sh.cn; luruiju62@163.com；Tel: 021-62201032; 021-62202965

摘　要：

采用单花滴注法将禾谷镰刀菌分生孢子接种42份大麦品种(系), 对其赤霉病菌的抗性进行评价, 并利用DON毒素对抗性不同大麦的小孢子进行离体胁迫培养。结果表明, 供试品种(系)对赤霉病抗性存在明显差异, 可将其划分为高抗、中抗、中感和重感四种类型。在5 mg•L^-1 DON毒素的胁迫下, 抗性不同的大麦小孢子的愈伤组织相对产量为高抗>中抗>高感, 表明供试品种小孢子水平的耐DON毒素能力与植株水平的抗赤霉病能力之间存在一定的联系。

关键词：大麦; 赤霉病; DON毒素; 小孢子; 愈伤组织相对产量

收稿：2015-10-14   修定：2015-11-12

资助：上海市科委基础研究项目(14JC1405300)、上海市农委成长计划[沪农青字(2015)第1-29号]和大麦青稞产业技术体系(CARS-05)。

Evaluation of the Resistance of Barley Varieties (Lines) to Fusarium Head Blight and Responses of Isolated Microspores to Deoxynivalenol Stress

GAO Run-Hong^1,2, GUO Gui-Mei^1,2, HE Ting^1,2, CHEN Zhi-Wei^1,2, XU Hong-Wei^1,2, LI Ying-Bo^1,2, LIU Cheng-Hong^1,2, LU Rui-Ju^1,2,*, HUANG Jian-Hua^1,2,*

¹Biotech Research Institute, Shanghai Academy of Agricultural Sciences, Shanghai 201106, China; ²Shanghai Key Laboratory of Agricultural Genetics and Breeding, Shanghai 201106, China

Corresponding author: LU Rui-Ju; E-mail: sw1@saas.sh.cn; luruiju62@163.com; Tel: 021-62201032; 021-62202965

Abstract:

The Fusarium head blight (FHB) resistance of different barley varieties (lines) were investigated by single floret injection. The results showed that there were significant differences among barley varieties (lines) for FHB resistance, which could be categorized into four groups: highly resistant, moderately resistant, moderately susceptible and highly susceptible. Responses of barley isolated microspores cultured by deoxynivalenol (DON) treatment were further investigated. The relative callus yields from isolated microspores of barley varieties (lines) with different levels of FHB resistance were obviously different. When cultured in induction medium supplemented by 5 mg•L^-1 DON, highly resistant line had the highest yield, followed by moderately resistant line, and highly susceptible had the lowest yield. These results suggested that the response of cultured microspores to DON stress had correlation with FHB resistance.

Key words: barley; Fusarium head blight (FHB); deoxynivalenol; microspore; relative callus yield